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KMID : 0616120200510020107
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Korean Journal of Pharmacognosy
2020 Volume.51 No. 2 p.107 ~ p.114
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Anti-inflammatory Effect of p-Hydroxycinnamic Acid on HT-29 Intestinal Cells and Its Therapeutic Effect of Immune Bowel Disease
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Lee Hyun-Su
Lee Seung-Ho
Choi Hyuk-Jae
Jeong Gil-Saeng
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Abstract
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Inflammatory bowel disease (IBD) is a chronic inflammatory disorder on the large intestine that has been considered as an incurable not only in Western society but also in Eastern Asia in recent years. Despite enormous efforts to develop novel therapeutics for this disease, strategy using bioactive compounds from natural product is still considered as important. p-hydroxycinnamic acid (HCA) is an intermediate substance found in several plants and has been known to possess anti-inflammation but little evidence is reported whether HCA has an inhibitory effect on intestinal inflammation. In the present study, we observed HCA does not show cytotoxic and apoptotic in HT-29 cells. Quantitative PCR analysis revealed that HCA effectively blocks the activity of HT-29 cells stimulated with TNF-a treatment. HCA inhibits translocation of p65 and MAPK pathways in activated HT-29 cells by TNF-a treatment. Besides, oral administration of HCA attenuates manifestation of DSS-induced inflammatory disease in vivo. Histological analysis exhibited that oral administration of HCA recovers IBD symptoms. The expression of pro-inflammatory cytokines were reduced by oral administration of HCA on intestinal tissues. Therefore, these results suggest that HCA has a potent anti-inflammatory effect on intestinal cells as well as show a therapeutic potential for treating IBD in vivo.
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KEYWORD
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p-Hydroxycinnamic acid, HT-29, TNF-a, MAPK, Immune bowel disease, Anti-inflammation
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